Enhanced 2,3-butanediol production in recombinant Klebsiella pneumoniae via overexpression of synthesis-related genes.

@article{Kim2012Enhanced2P,
  title={Enhanced 2,3-butanediol production in recombinant Klebsiella pneumoniae via overexpression of synthesis-related genes.},
  author={Borim Kim and Soojin Lee and Joo-Ung Park and Mingshou Lu and Min-kyu Oh and Young-Rok Kim and Jinwon Lee},
  journal={Journal of microbiology and biotechnology},
  year={2012},
  volume={22 9},
  pages={
          1258-63
        }
}
2,3-Butanediol (2,3-BD) is a major metabolite produced by Klebsiella pneumoniae KCTC2242, which is a important chemical with wide applications. Three genes important for 2,3-BD biosynthesis acetolactate decarboxylase (budA), acetolactate synthase (budB), and alcohol dehydrogenase (budC) were identified in K. pneumoniae genomic DNA. With the goal of enhancing 2,3-BD production, these genes were cloned into pUC18K expression vectors containing the lacZ promoter and the kanamycin resistance gene… 
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Industrial Production of 2,3-Butanediol from the Engineered Corynebacterium glutamicum
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Production of 2,3-BDO with the non-pathogenic and rapidly growing Corynebacterium glutamicum is produced and budA encoding acetolactate decarboxylase from Klebsiella pneumoniae is introduced, a powerful 2, 3- BDO producer.
Observation of 2,3-butanediol biosynthesis in Lys regulator mutated Klebsiella pneumoniae at gene transcription level.
The regulation of 2,3-butanediol synthesis in Klebsiella pneumoniae as revealed by gene over-expressions and metabolic flux analysis
TLDR
The MFA results showed that the seven overexpressed mutants all exhibited enhanced 2,3-BDO production, and the strain overexpressing the budBA gene produced the highest yield.
Improvement of 2,3-Butanediol Yield in Klebsiella pneumoniae by Deletion of the Pyruvate Formate-Lyase Gene
TLDR
From an analysis of the transcriptome, it was confirmed that the removal of pflB from K. pneumoniae significantly repressed the expression of genes involved in the formate hydrogen lyase (FHL) system.
Redistribution of Carbon Flux toward 2,3-Butanediol Production in Klebsiella pneumoniae by Metabolic Engineering
TLDR
Data on batch culture and gene transcription provide insight into improving the metabolic network for 2,3-BDO biosynthesis for industrial applications.
Selective Production of 2,3-Butanediol and Acetoin by a Newly Isolated Bacterium Klebsiella oxytoca M1
TLDR
The results in this study demonstrate the feasibility of using K. oxytoca M1 for the production of not only 2,3-BDO but also acetoin as a major product.
Efficient production of 2,3-butanediol by recombinant Saccharomyces cerevisiae through modulation of gene expression by cocktail δ-integration.
The influence of budA deletion on glucose metabolism related in 2,3-butanediol production by Klebsiella pneumoniae.
Deletion of the budBAC operon in Klebsiella pneumoniae to understand the physiological role of 2,3-butanediol biosynthesis
TLDR
This study provides a prime insight into the role of 2,3-BDO biosynthesis of K. pneumoniae by constructed a budBAC-deleted strain and observed significant differences in the production of extracellular metabolites, CO2 emission, carbon distribution, and NADH/NAD+.
Enhanced 2,3-Butanediol Production by Optimizing Fermentation Conditions and Engineering Klebsiella oxytoca M1 through Overexpression of Acetoin Reductase
TLDR
High production of 2,3-BDO using a previously isolated bacterium Klebsiella oxytoca M1 was carried out by optimizing fermentation conditions and overexpressing acetoin reductase (AR); supplying complex nitrogen sources and using NaOH as a neutralizing agent were found to enhance specific production and yield.
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