Engrailed Negatively Regulates the Expression of Cell Adhesion Molecules Connectin and Neuroglian in Embryonic Drosophila Nervous System

@article{Siegler1999EngrailedNR,
  title={Engrailed Negatively Regulates the Expression of Cell Adhesion Molecules Connectin and Neuroglian in Embryonic Drosophila Nervous System},
  author={M. Siegler and X. Jia},
  journal={Neuron},
  year={1999},
  volume={22},
  pages={265-276}
}
Engrailed is expressed in subsets of interneurons that do not express Connectin or appreciable Neuroglian, whereas other neurons that are Engrailed negative strongly express these adhesion molecules. Connectin and Neuroglian expression are virtually eliminated in interneurons when engrailed expression is driven ubiquitously in neurons, and greatly increased when engrailed genes are lacking in mutant embryos. The data suggest that Engrailed is normally a negative regulator of Connectin and… Expand
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References

SHOWING 1-10 OF 106 REFERENCES
Mutations in the Drosophila neuroglian cell adhesion molecule affect motor neuron pathfinding and peripheral nervous system patterning.
TLDR
Examination of the nervous systems of neuroglian mutant embryos reveals that motoneurons have altered pathfinding trajectories and the sensory cell bodies of the peripheral nervous system display altered morphology and patterning. Expand
Modifying expression of the engrailed gene of Drosophila melanogaster.
TLDR
Using P-element-mediated transposition, flies that express the engrailed gene under the control of an hsp70 promoter are generated, and it is found that ectopic, heat-shock-induced, engraile expression caused pattern defects similar to those in embryos lacking engrailing function. Expand
The Drosophila hedgehog gene is expressed specifically in posterior compartment cells and is a target of engrailed regulation.
TLDR
Sequence analysis reveals a motif characteristic of a transmembrane domain, suggesting that the hh protein is membrane-associated, and discusses the relationship between en and hh, and the role of the hH function. Expand
ming is expressed in neuroblast sublineages and regulates gene expression in the Drosophila central nervous system.
TLDR
It is proposed that ming controls cell fate within neuroblast cell lineages, which encodes a predicted zinc finger protein and results in precise alterations in CNS gene expression, defects in axonogenesis and embryonic lethality. Expand
Drosophila neuroglian: A member of the immunoglobulin superfamily with extensive homology to the vertebrate neural adhesion molecule L1
Drosophila neuroglian is an integral membrane glycoprotein that is expressed on a variety of cell types in the Drosophila embryo, including expression on a large subset of glial and neuronal cellExpand
The cell adhesion molecule, connectin, and the development of the Drosophila neuromuscular system.
TLDR
The timing and pattern of connectin expression suggest that it may play an important role in mediating specific interactions through homotypic cell adhesion through homotypesic cell-cell adhesion. Expand
The Drosophila islet Gene Governs Axon Pathfinding and Neurotransmitter Identity
TLDR
Findings indicate that Drosophila islet coordinately controls two distinct aspects of neuronal identity, specifically alterations in pathfinding and changes in neurotransmitter identity. Expand
beta3-tubulin is directly repressed by the engrailed protein in Drosophila.
TLDR
The identification of an effector gene, the beta3-tubulin gene, as a direct target of Engrailed is reported here, and two different sets ofEngrailed binding sites are shown to be involved in the early and late regulation of beta3 -tubulin by Engrailing during embryogenesis. Expand
Expression of engrailed proteins in arthropods, annelids, and chordates.
TLDR
A monoclonal antibody is described that recognizes a conserved epitope in the homeodomain of engrailed proteins of a number of different arthropods, annelids, and chordates; this antibody is used to isolate the grasshopperEngrailed gene, a homeobox gene that has an important role in Drosophila segmentation. Expand
Connectin Mediates Adhesion in Drosophila
TLDR
It is demonstrated that connectin mutants do have a phenotype; the normally connectin-positive pleural muscles fail to adhere closely together, and it is argued that the phenotypes are consistent with connectin's adhesion role. Expand
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