Engineering unnatural nucleotide specificity to probe G protein signaling.

  title={Engineering unnatural nucleotide specificity to probe G protein signaling.},
  author={Fabien Vincent and Silas P Cook and Emmanuel Johnson and Dana Emmert and Kavita Shah},
  journal={Chemistry & biology},
  volume={14 9},
G proteins comprise approximately 0.5% of proteins encoded by mammalian genomes. To date, there exists a lack of small-molecule modulators that could contribute to their functional study. In this report, we present the use of H-Ras to develop a system that answers this need. Small molecules that allow for the highly specific inhibition or activation of the engineered G protein were developed. The rational design preserved binding of the natural substrates to the G protein, and the mutations… CONTINUE READING