Engineering transcription factors with novel DNA-binding specificity using comparative genomics

@inproceedings{Desai2009EngineeringTF,
  title={Engineering transcription factors with novel DNA-binding specificity using comparative genomics},
  author={Tasha A. Desai and Dmitry A. Rodionov and Mikhail S. Gelfand and Eric John Alm and Christopher V. Rao},
  booktitle={Nucleic acids research},
  year={2009}
}
The transcriptional program for a gene consists of the promoter necessary for recruiting RNA polymerase along with neighboring operator sites that bind different activators and repressors. From a synthetic biology perspective, if the DNA-binding specificity of these proteins can be changed, then they can be used to reprogram gene expression in cells. While many experimental methods exist for generating such specificity-altering mutations, few computational approaches are available, particularly… CONTINUE READING

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