Engineering the thermostability of Trichoderma reesei endo-1,4-β-xylanase II by combination of disulphide bridges

@article{Xiong2004EngineeringTT,
  title={Engineering the thermostability of Trichoderma reesei endo-1,4-β-xylanase II by combination of disulphide bridges},
  author={Hairong Xiong and Fred Fenel and Matti S. A. Leisola and Ossi Turunen},
  journal={Extremophiles},
  year={2004},
  volume={8},
  pages={393-400}
}
Disulphide bridges were introduced in different combinations into the N-terminal region and the single α-helix of mesophilic Trichoderma reesei xylanase II (TRX II). We used earlier disulphide-bridge data and designed new disulphide bridges for the combination mutants. The most stable mutant contained two disulphide bridges (between positions 2 and 28 and between positions 110 and 154, respectively) and the mutations N11D, N38E, and Q162H. With a half-life of ~56 h at 65°C, the thermostability… CONTINUE READING