Engineering the substrate specificity of xylose isomerase.

  title={Engineering the substrate specificity of xylose isomerase.},
  author={Johanna Karim{\"a}ki and Tarja Parkkinen and H. S. Dalla Santa and Ossi A Pastinen and Matti S. A. Leisola and Juha Rouvinen and Ossi Turunen},
  journal={Protein engineering, design & selection : PEDS},
  volume={17 12},
Xylose isomerase (XI) catalyzes the isomerization and epimerization of hexoses, pentoses and tetroses. In order to clarify the reasons for the low reaction efficiency of a pentose sugar, L-arabinose, we determined the crystal structure of Streptomyces rubiginosus XI complexed with L-arabinose. The crystal structure revealed that, when compared with D-xylose and D-glucose, L-arabinose binds to the active site in a partially different position, in which the ligand has difficulties in binding the… CONTINUE READING
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