Engineering the substrate specificity of Alcaligenes D-aminoacylase useful for the production of D-amino acids by optical resolution.

@article{Yano2011EngineeringTS,
  title={Engineering the substrate specificity of Alcaligenes D-aminoacylase useful for the production of D-amino acids by optical resolution.},
  author={Shigekazu Yano and Hiroyuki Haruta and Takuya Ikeda and Takeshi Kikuchi and Masahiro Murakami and Mitsuaki Moriguchi and Mamoru Wakayama},
  journal={Journal of chromatography. B, Analytical technologies in the biomedical and life sciences},
  year={2011},
  volume={879 29},
  pages={3247-52}
}
D-Aminoacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6 (AxD-NAase) offers a novel biotechnological application, the production of D-amino acid from the racemic mixture of N-acyl-DL-amino acids. However, its substrate specificity is biased toward certain N-acyl-D-amino acids. To construct mutant AxD-NAases with substrate specificities different from those of wild-type enzyme, the substrate recognition site of the AxD-NAase was rationally manipulated based on computational… CONTINUE READING
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