Engineering the root-soil interface via targeted expression of a synthetic phytase gene in trichoblasts.

@article{Zimmermann2003EngineeringTR,
  title={Engineering the root-soil interface via targeted expression of a synthetic phytase gene in trichoblasts.},
  author={Philip Zimmermann and Gerardo I Zardi and Martin Lehmann and Christophe Zeder and Nikolaus Amrhein and Emmanuel Frossard and Marcel Bucher},
  journal={Plant biotechnology journal},
  year={2003},
  volume={1 5},
  pages={353-60}
}
For biochemical modification of the root-soil interface, the engineered secretion of stable enzymes from trichoblasts (= root hair bearing rhizodermal cells) is proposed. As a reporter activity, we chose to express a synthetic gene encoding a secretory phytase (PHY) directed by a trichoblast-specific promoter in root hair cells of the crop plant potato. Transgenic plants produced and secreted phytase in sufficient amounts to release phosphate from phytate in liquid medium. When grown in an… CONTINUE READING

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