Engineering the push and pull of lipid biosynthesis in oleaginous yeast Yarrowia lipolytica for biofuel production.

@article{Tai2013EngineeringTP,
  title={Engineering the push and pull of lipid biosynthesis in oleaginous yeast Yarrowia lipolytica for biofuel production.},
  author={Mitchell Tai and Gregory Stephanopoulos},
  journal={Metabolic engineering},
  year={2013},
  volume={15},
  pages={1-9}
}
Microbial oil production by heterotrophic organisms is a promising path for the cost-effective production of biofuels from renewable resources provided high conversion yields can be achieved. To this end, we have engineered the oleaginous yeast Yarrowia lipolytica. We first established an expression platform for high expression using an intron-containing translation elongation factor-1α (TEF) promoter and showed that this expression system is capable of increasing gene expression 17-fold over… CONTINUE READING
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