Engineering the genome of Thermus thermophilus using a counterselectable marker.

  title={Engineering the genome of Thermus thermophilus using a counterselectable marker.},
  author={Jennifer F. Carr and Michael E Danziger and Athena L. Huang and Albert E. Dahlberg and Steven T. Gregory},
  journal={Journal of bacteriology},
  volume={197 6},
UNLABELLED Thermus thermophilus is an extremely thermophilic bacterium that is widely used as a model thermophile, in large part due to its amenability to genetic manipulation. Here we describe a system for the introduction of genomic point mutations or deletions using a counterselectable marker consisting of a conditionally lethal mutant allele of pheS encoding the phenylalanyl-tRNA synthetase α-subunit. Mutant PheS with an A294G amino acid substitution renders cells sensitive to the… CONTINUE READING
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