Engineering the Pichia pastoris methanol oxidation pathway for improved NADH regeneration during whole-cell biotransformation.

@article{Schroer2010EngineeringTP,
  title={Engineering the Pichia pastoris methanol oxidation pathway for improved NADH regeneration during whole-cell biotransformation.},
  author={Kirsten Schroer and Klaus Peter Luef and Franz Stefan Hartner and Anton Glieder and Beate Pscheidt},
  journal={Metabolic engineering},
  year={2010},
  volume={12 1},
  pages={8-17}
}
Industrial biocatalytic reduction processes require the efficient regeneration of reduced cofactors for the asymmetric reduction of prochiral compounds to chiral intermediates which are needed for the production of fine chemicals and drugs. Here, we present a new engineering strategy for improved NADH regeneration based on the Pichia pastoris methanol oxidation pathway. Studying the kinetic properties of alcohol oxidase (AOX), formaldehyde dehydrogenase (FLD) and formate dehydrogenase (FDH) and… CONTINUE READING

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