Engineering proteins, subcloning and hyperexpressing oxidoreductase genes.

@article{Darwish1991EngineeringPS,
  title={Engineering proteins, subcloning and hyperexpressing oxidoreductase genes.},
  author={Kamel Darwish and Hong Li and Thomas L Poulos},
  journal={Protein engineering},
  year={1991},
  volume={4 6},
  pages={701-8}
}
A very efficient system for subcloning and studying protein sequences, combining previously established elements for hyperexpression, replication and screening, was used to hyperproduce and characterize seven different products. It expedited the cloning of genes, in a multipurpose recombinant DNA construct, for all the requirements to study and engineer… CONTINUE READING