Engineering plant disease resistance based on TAL effectors.

@article{Schornack2013EngineeringPD,
  title={Engineering plant disease resistance based on TAL effectors.},
  author={Sebastian Schornack and Matthew J. Moscou and Eric Russell Ward and Diana M. Horvath},
  journal={Annual review of phytopathology},
  year={2013},
  volume={51},
  pages={
          383-406
        }
}
Transcription activator-like (TAL) effectors are encoded by plant-pathogenic bacteria and induce expression of plant host genes. TAL effectors bind DNA on the basis of a unique code that specifies binding of amino acid residues in repeat units to particular DNA bases in a one-to-one correspondence. This code can be used to predict binding sites of natural TAL effectors and to design novel synthetic DNA-binding domains for targeted genome manipulation. Natural mechanisms of resistance in plants… 
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Two ancestral genes shaped the Xanthomonas campestris TAL effector gene repertoire.
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References

SHOWING 1-10 OF 155 REFERENCES
TAL effectors: finding plant genes for disease and defense.
RNA-seq pinpoints a Xanthomonas TAL-effector activated resistance gene in a large-crop genome
TLDR
It is demonstrated that TALE-specific R genes can be cloned from large-genome crops with a highly efficient RNA-seq approach and are as tightly regulated as pepper Bs4C.
A single plant resistance gene promoter engineered to recognize multiple TAL effectors from disparate pathogens
TLDR
Recognition of AvrBs3 was shown to be mediated by interaction with the corresponding UPT (UPregulated by TAL effectors) box UPTAvrBs3 present in the promoter R gene Bs3 from the dicot pepper, but it was not known how the Xoo Tal effector AvrXa27 transcriptionally activates the matching R gene Xa27 from the monocot rice.
A Bacterial Effector Acts as a Plant Transcription Factor and Induces a Cell Size Regulator
TLDR
It is shown that AvrBs3 induces the expression of a master regulator of cell size, upa20, which encodes a transcription factor containing a basic helix-loop-helix domain that provokes developmental reprogramming of host cells by mimicking eukaryotic transcription factors.
TAL effectors: function, structure, engineering and applications.
Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors
TLDR
The functionality of a distinct type of DNA binding domain is described and allows the design ofDNA binding domains for biotechnology.
Xanthomonas AvrBs3 family-type III effectors: discovery and function.
TLDR
The discovery of TAL effectors is described, which act as transcriptional activators in the plant cell nucleus and are determined by a novel modular DNA-binding domain.
Plant Pathogen Recognition Mediated by Promoter Activation of the Pepper Bs3 Resistance Gene
TLDR
The data suggest a recognition mechanism in which the Avr protein binds and activates the promoter of the cognate R gene, and a recognition specificity resides in the Bs3 and Bs2-E promoters and is determined by binding of Avr3 or AvrBs3Δrep16 to a defined promoter region.
Addition of transcription activator-like effector binding sites to a pathogen strain-specific rice bacterial blight resistance gene makes it effective against additional strains and against bacterial leaf streak.
TLDR
Six TAL effector binding elements were added to the rice Xa27 gene and Sequences in the EBEs were found to occur nonrandomly in rice promoters, suggesting an overlap with endogenous regulatory sequences, suggesting executor gene specificity can be broadened by adding E BEs, but caution is warranted because of the possible coincident introduction of endogenous regulatory elements.
R gene expression induced by a type-III effector triggers disease resistance in rice
TLDR
Xa 27 specificity towards incompatible pathogens involves the differential expression of the R gene in the presence of the AvrXa27 effector.
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