Engineering of the H2O2-binding pocket region of a recombinant manganese peroxidase to be resistant to H2O2.

@article{Miyazaki2001EngineeringOT,
  title={Engineering of the H2O2-binding pocket region of a recombinant manganese peroxidase to be resistant to H2O2.},
  author={Chie Miyazaki and Haruo Takahashi},
  journal={FEBS letters},
  year={2001},
  volume={509 1},
  pages={111-4}
}
The manganese peroxidase produced by Phanerochaete chrysosporium, which catalyzes the oxidation of Mn(2+) to Mn(3+), is easily inactivated by the hydrogen peroxide (H2O2) presented in the reaction. We attempted to increase H2O2 resistance by the conformational stabilization around the H2O2-binding pocket. Based on its structural model, engineering of oxidizable Met273 located near the pocket to a non-oxidizable Leu showed a great improvement. Furthermore, after treatment at 1 mM H2O2 where the… CONTINUE READING

From This Paper

Topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 13 extracted citations

Similar Papers

Loading similar papers…