Engineering of plasmin-resistant forms of streptokinase and their production in Bacillus subtilis: streptokinase with longer functional half-life.

@article{Wu1998EngineeringOP,
  title={Engineering of plasmin-resistant forms of streptokinase and their production in Bacillus subtilis: streptokinase with longer functional half-life.},
  author={Xiaomiao Wu and R. Li Ye and Yanjun Duan and Sandra L Wong},
  journal={Applied and environmental microbiology},
  year={1998},
  volume={64 3},
  pages={824-9}
}
The short in vivo half-life of streptokinase limits its efficacy as an efficient blood clot-dissolving agent. During the clot-dissolving process, streptokinase is processed to smaller intermediates by plasmin. Two of the major processing sites are Lys59 and Lys386. We engineered two versions of streptokinase with either one of the lysine residues changed to glutamine and a third version with both mutations. These mutant streptokinase proteins (muteins) were produced by secretion with the… CONTINUE READING