Engineering of high yield production of L-serine in Escherichia coli.

  title={Engineering of high yield production of L-serine in Escherichia coli.},
  author={Hemanshu Mundhada and Konstantin Schneider and Hanne Bjerre Christensen and Alex Toftgaard Nielsen},
  journal={Biotechnology and bioengineering},
  volume={113 4},
L-serine is a widely used amino acid that has been proposed as a potential building block biochemical. The high theoretical yield from glucose makes a fermentation based production attractive. In order to achieve this goal, serine degradation to pyruvate and glycine in E. coli MG1655 was prevented by deletion of three L-serine deaminases sdaA, sdaB, and tdcG, as well as serine hydroxyl methyl transferase (SHMT) encoded by glyA. Upon overexpression of the serine production pathway, consisting of… CONTINUE READING


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