Engineering of core promoter regions enables the construction of constitutive and inducible promoters in Halomonas sp.

@article{Li2016EngineeringOC,
  title={Engineering of core promoter regions enables the construction of constitutive and inducible promoters in Halomonas sp.},
  author={Tingting Li and Teng Li and Weiyue Ji and Qiuyue Wang and Haoqian M Zhang and Guoqiang Chen and Chunbo Lou and Qi Ouyang},
  journal={Biotechnology Journal},
  year={2016},
  volume={11}
}
Halomonas strain TD01, a newly identified halophilic bacterium, has proven to be a promising low‐cost host for the production of chemicals. However, genetic manipulation in Halomonas sp. is still difficult due to the lack of well‐characterized and tunable expression systems. In this study, a systematic, efficient method was exploited to construct both a constitutive promoter library and inducible promoters. Porin, a highly expressed protein in Halomonas TD01, was first identified from the… 
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By substituting the ectA promoter with a promoter P265 that identified in the outer membrane pore protein E of H. hydrothermalis Y2, the salt dependence of ectoine synthesis was significantly decreased and this promoter engineering strategy provides a practical protocol for lowering the salt-dependence of ect Frenchman synthesis in this moderately halotolerant strain.
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