Engineering of class I lactate-polymerizing polyhydroxyalkanoate synthases from Ralstonia eutropha that synthesize lactate-based polyester with a block nature

@article{Ochi2012EngineeringOC,
  title={Engineering of class I lactate-polymerizing polyhydroxyalkanoate synthases from Ralstonia eutropha that synthesize lactate-based polyester with a block nature},
  author={Anna Ochi and Ken’ichiro Matsumoto and Takashi Ooba and Kohei Sakai and Takeharu Tsuge and Seiichi Taguchi},
  journal={Applied Microbiology and Biotechnology},
  year={2012},
  volume={97},
  pages={3441-3447}
}
Class I polyhydroxyalkanoate (PHA) synthase from Ralstonia eutropha (PhaCRe) was engineered so as to acquire an unusual lactate (LA)-polymerizing activity. To achieve this, the site-directed saturation mutagenesis of PhaCRe was conducted at position 510, which corresponds to position 481 in the initially discovered class II LA-polymerizing PHA synthase… CONTINUE READING