Engineering of citrus to obtain huanglongbing resistance.

@article{Alquzar2021EngineeringOC,
  title={Engineering of citrus to obtain huanglongbing resistance.},
  author={Berta Alqu{\'e}zar and Lourdes Carmona and Stefania Bennici and Leandro Pe{\~n}a},
  journal={Current opinion in biotechnology},
  year={2021},
  volume={70},
  pages={
          196-203
        }
}
2 Citations
Generation of Transfer-DNA-Free Base-Edited Citrus Plants
TLDR
Analysis of transgene-free plants indicated that the transient expression of the T- DNA genes was sufficient to induce ALS mutations and thus generate IMZ-resistant shoots at 11.7% frequency, which is the first report of T-DNA-free edited citrus plants.

References

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Long-Term Field Evaluation Reveals Huanglongbing Resistance in Citrus Relatives.
TLDR
Disease resistance/tolerance was observed in Australian citrus relative genera Eremocitrus and Microcitrus, which are sexually compatible with citrus and may be useful in future breeding trials to impart HLB resistance to cultivated citrus.
Overexpression of a Modified Plant Thionin Enhances Disease Resistance to Citrus Canker and Huanglongbing (HLB)
TLDR
The results showed significant Las titer reduction in roots of transgenic Carrizo compared with control plants and reduced scionLas titer 12 months after graft inoculation provide promise for engineering citrus disease resistance against HLB and canker.
An effector from the Huanglongbing-associated pathogen targets citrus proteases
TLDR
It is shown that an effector cloned from the associated bacteria can suppress host plant papain-like cysteine proteases' activity, suggesting its probable role in pathogenesis.
Overview of citrus huanglongbing spread and management strategies in Brazil
TLDR
The management of HLB in São Paulo and Triângulo/Sudoeste Mineiro citrus belt has been considered a success case and has ensured the maintenance of citrus production and competitiveness of the Brazilian citrus industry while new, more durable, and sustainable measures are not yet available.
Root samples provide early and improved detection of Candidatus Liberibacter asiaticus in Citrus
TLDR
A longitudinal study of young asymptomatic trees was established to observe the spread of disease through time and test the relative effectiveness of leaf- and root-based detection strategies.
A stable antimicrobial peptide with dual functions of treating and preventing citrus Huanglongbing
TLDR
A novel class of stable antimicrobial peptides (SAMPs) from Australian finger lime and other HLB-tolerant citrus close relatives is identified, which has dual functions of inhibiting CLas growth in H LB-positive trees and activating host immunity to prevent new infections.
Overexpression of the Arabidopsis NPR1 protein in citrus confers tolerance to Huanglongbing
TLDR
Results demonstrate that overexpression of the AtNPR1 protein in citrus is able to provide robust tolerance to HLB, and find that the same three transgenic lines are resistant to citrus canker.
Understanding the Physiology of Liberibacter asiaticus: An Overview of the Demonstrated Molecular Mechanisms
TLDR
The scientific progress made by studying L. asiaticus’ biology through direct experimentation is summarized and it is imperiously necessary to increase the basic research to identify relevant biological clues to control citrus greening.
Repellency of forty‐one aromatic plant species to the Asian citrus psyllid, vector of the bacterium associated with huanglongbing
TLDR
The results showed that these aromatic species, being highly repellent to ACP, offer great potential as more cost‐effective and environmentally sustainable alternatives to the current methods of managing HLB.
Engineered Orange Ectopically Expressing the Arabidopsis β-Caryophyllene Synthase Is Not Attractive to Diaphorina citri, the Vector of the Bacterial Pathogen Associated to Huanglongbing
TLDR
Genetically modified sweet orange lines are generated to constitutively emit (E)-β-caryophyllene, a sesquiterpene repellent to D. citri, the main HLB psyllid vector, opening a new alternative for possible HLB control in the field.
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