Engineering of chicken avidin: a progressive series of reduced charge mutants.

@article{Marttila1998EngineeringOC,
  title={Engineering of chicken avidin: a progressive series of reduced charge mutants.},
  author={Ari T Marttila and Kari J. Airenne and Olli H. Laitinen and Tikva Kulik and Edward A Bayer and Meir Wilchek and Markku S. Kulomaa},
  journal={FEBS letters},
  year={1998},
  volume={441 2},
  pages={313-7}
}
Avidin, a positively charged egg-white glycoprotein, is a widely used tool in biotechnological applications because of its ability to bind biotin strongly. The high pI of avidin (approximately 10.5), however, is a hindrance in certain applications due to non-specific (charge-related) binding. Here we report a construction of a series of avidin charge mutants with pIs ranging from 9.4 to 4.7. Rational design of the avidin mutants was based on known crystallographic data together with comparative… CONTINUE READING