Engineering of biotin-prototrophy in Pichia pastoris for robust production processes.

Abstract

Biotin plays an essential role as cofactor for biotin-dependent carboxylases involved in essential metabolic pathways. The cultivation of Pichia pastoris, a methylotrophic yeast that is successfully used as host for the production of recombinant proteins, requires addition of high dosage of biotin. As biotin is the only non-salt media component used during… (More)
DOI: 10.1016/j.ymben.2010.07.002

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