Engineering of a novel biochemical pathway for the biosynthesis of L-2-aminobutyric acid in Escherichia coli K12.

@article{Fotheringham1999EngineeringOA,
  title={Engineering of a novel biochemical pathway for the biosynthesis of L-2-aminobutyric acid in Escherichia coli K12.},
  author={Ian Graham Fotheringham and Nigel Grinter and David P. Pantaleone and Richard F Senkpeil and P P Taylor},
  journal={Bioorganic & medicinal chemistry},
  year={1999},
  volume={7 10},
  pages={
          2209-13
        }
}
L-2-Aminobutyric acid was synthesised in a transamination reaction from L-threonine and L-aspartic acid as substrates in a whole cell biotransformation using recombinant Escherichia coli K12. The cells contained the cloned genes tyrB, ilvA and alsS which respectively encode tyrosine aminotransferase of E. coli, threonine deaminase of E. coli and alpha-acetolactate synthase of B. subtilis 168. The 2-aminobutyric acid was produced by the action of the aminotransferase on 2-ketobutyrate and L… CONTINUE READING

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Removal of l-alanine from the production of l-2-aminobutyric acid by introduction of alanine racemase and d-amino acid oxidase

  • Applied Microbiology and Biotechnology
  • 2011
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