Engineering of a monomeric green-to-red photoactivatable fluorescent protein induced by blue light

@article{Gurskaya2006EngineeringOA,
  title={Engineering of a monomeric green-to-red photoactivatable fluorescent protein induced by blue light},
  author={Nadya G. Gurskaya and Vladislav V Verkhusha and Alexander S Shcheglov and Dmitry B. Staroverov and Tatyana V Chepurnykh and Arkady F. Fradkov and Sergey A. Lukyanov and Konstantin A. Lukyanov},
  journal={Nature Biotechnology},
  year={2006},
  volume={24},
  pages={461-465}
}
Green fluorescent protein (GFP) and GFP-like proteins represent invaluable genetically encoded fluorescent probes. In the last few years a new class of photoactivatable fluorescent proteins (PAFPs) capable of pronounced light-induced spectral changes have been developed. Except for tetrameric KFP1 (ref. 4), all known PAFPs, including PA-GFP, Kaede, EosFP, PS-CFP, Dronpa, PA-mRFP1 and KikGR require light in the UV-violet spectral region for activation through one-photon excitation—such light can… CONTINUE READING
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