Engineering of Saccharomyces cerevisiae for the synthesis of short chain fatty acids

  title={Engineering of Saccharomyces cerevisiae for the synthesis of short chain fatty acids},
  author={Christopher Leber and Nancy A Da Silva},
  journal={Biotechnology and Bioengineering},
Carbon feedstocks from fossilized sources are being rapidly depleted due to rising demand for industrial and commercial applications. Many petroleum‐derived chemicals can be directly or functionally substituted with chemicals derived from renewable feedstocks. Several short chain organic acids may fulfill this role using their functional groups as a target for chemical catalysis. Saccharomyces cerevisiae was engineered to produce short chain carboxylic acids (C6 to C10) from glucose using the… 
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A metabolically engineered strain of Escherichia coli is reported that overproduces medium‐chain length fatty acids via three basic modifications: elimination of β‐oxidation, overexpression of the four subunits of acetyl‐CoA carboxylase, and expression of a plant acyl–acyl carrier protein (ACP) thioesterase from Umbellularia californica (BTE).
Overproduction of free fatty acids in E. coli: implications for biodiesel production.
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The results showed overexpressing ‘TesA and the deletion of fadL in E. coli BL21 (DE3) improved extracellular fatty acid production, while deletion offadD didn’t strengthen the extrace cellular fatty acids production for an undetermined mechanism, suggesting that the supposedly superior strain did not necessarily perform best for the efficient production of desired product.
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