Engineering of Rhodococcus cell catalysts for tolerance improvement by sigma factor mutation and active plasmid partition

@article{Ma2012EngineeringOR,
  title={Engineering of Rhodococcus cell catalysts for tolerance improvement by sigma factor mutation and active plasmid partition},
  author={Yuchao Ma and Huimin Yu},
  journal={Journal of Industrial Microbiology & Biotechnology},
  year={2012},
  volume={39},
  pages={1421-1430}
}
Tolerance to various stresses is a key phenotype for cell catalysts, which are used widely in bioproduction of diverse valuable chemicals. Using the Rhodococcus ruber TH strain, which exhibits high nitrile hydratase activity, as the target cell catalyst for acrylamide production, we established a method to improve cell tolerance by stably introducing global transcription perturbation. The σ70 gene (sigA) of R. ruber was cloned and randomly mutated. An R. ruber TH3/pNV-sigA M library containing… CONTINUE READING

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