Engineering of NADPH regenerators in Escherichia coli for enhanced biotransformation

@article{Lee2013EngineeringON,
  title={Engineering of NADPH regenerators in Escherichia coli for enhanced biotransformation},
  author={Wwm Lee and Myoungkeun Kim and Yong-Su Jin and Jin-Ho Seo},
  journal={Applied Microbiology and Biotechnology},
  year={2013},
  volume={97},
  pages={2761-2772}
}
Efficient regeneration of NADPH is one of the limiting factors that constrain the productivity of biotransformation processes. In order to increase the availability of NADPH for enhanced biotransformation by engineered Escherichia coli, modulation of the pentose phosphate pathway and amplification of the transhydrogenases system have been conventionally attempted as primary solutions. Recently, other approaches for stimulating NADPH regeneration during glycolysis, such as replacement of native… CONTINUE READING
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