Engineering of Family-5 Glycoside Hydrolase (Cel5A) from an Uncultured Bacterium for Efficient Hydrolysis of Cellulosic Substrates

@inproceedings{Telke2013EngineeringOF,
  title={Engineering of Family-5 Glycoside Hydrolase (Cel5A) from an Uncultured Bacterium for Efficient Hydrolysis of Cellulosic Substrates},
  author={Amar Anandrao Telke and Ningning Zhuang and Sunil Subhash Ghatge and Sook-Hee Lee and Asad Ali Shah and Haji Karim Khan and Youngsoon Um and Hyun-dong Shin and Young Ryun Chung and Kon Ho Lee and Seon-Won Kim},
  booktitle={PloS one},
  year={2013}
}
Cel5A, an endoglucanase, was derived from the metagenomic library of vermicompost. The deduced amino acid sequence of Cel5A shows high sequence homology with family-5 glycoside hydrolases, which contain a single catalytic domain but no distinct cellulose-binding domain. Random mutagenesis and cellulose-binding module (CBM) fusion approaches were successfully applied to obtain properties required for cellulose hydrolysis. After two rounds of error-prone PCR and screening of 3,000 mutants, amino… CONTINUE READING

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