Engineering of Corynebacterium glutamicum for high-yield L-valine production under oxygen deprivation conditions.

@article{Hasegawa2013EngineeringOC,
  title={Engineering of Corynebacterium glutamicum for high-yield L-valine production under oxygen deprivation conditions.},
  author={Satoshi Hasegawa and Masako Suda and Kimio Uematsu and Yumi Natsuma and Kazumi Hiraga and Toru Jojima and Masayuki Inui and Hideaki Yukawa},
  journal={Applied and environmental microbiology},
  year={2013},
  volume={79 4},
  pages={
          1250-7
        }
}
We previously demonstrated efficient L-valine production by metabolically engineered Corynebacterium glutamicum under oxygen deprivation. To achieve the high productivity, a NADH/NADPH cofactor imbalance during the synthesis of l-valine was overcome by engineering NAD-preferring mutant acetohydroxy acid isomeroreductase (AHAIR) and using NAD-specific leucine dehydrogenase from Lysinibacillus sphaericus. Lactate as a by-product was largely eliminated by disrupting the lactate dehydrogenase gene… CONTINUE READING

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Application of metabolic engineering for the biotechnological production of l-valine

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Production of l-valine from metabolically engineered Corynebacterium glutamicum

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