Engineering membrane protein overproduction in Escherichia coli.

  title={Engineering membrane protein overproduction in Escherichia coli.},
  author={Daniel Martinez Molina and Tobias Carl Cornvik and Said Eshaghi and Jesper Z. Haeggstr{\"o}m and Paer Nordlund and Marina Ignatushchenko Sabet},
  journal={Protein science : a publication of the Protein Society},
  volume={17 4},
Membrane proteins play a fundamental role in human disease and therapy, but suffer from a lack of structural and functional information compared to their soluble counterparts. The paucity of membrane protein structures is primarily due to the unparalleled difficulties in obtaining detergent-solubilized membrane proteins at sufficient levels and quality. We have developed an in vitro evolution strategy for optimizing the levels of detergent-solubilized membrane protein that can be overexpressed… CONTINUE READING

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