Engineering mRNA stability in E. coli by the addition of synthetic hairpins using a 5' cassette system.

@article{Carrier1997EngineeringMS,
  title={Engineering mRNA stability in E. coli by the addition of synthetic hairpins using a 5' cassette system.},
  author={Tabitha Carrier and Jay D Keasling},
  journal={Biotechnology and bioengineering},
  year={1997},
  volume={55 3},
  pages={577-80}
}
An expression system has been developed for the introduction of DNA cassettes into the region between the transcription and translation start sites of a gene of interest. This cassette system was used to engineer mRNA stability through the introduction of hairpins at the 5' end. A synthetic DNA cassette was designed so that the resulting mRNA hairpin would be positioned one nucleotide from the 5' mRNA end. The hairpin-containing mRNA exhibited a half-life 3 times that of the mRNA with no… CONTINUE READING

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