Engineering light-inducible nuclear localization signals for precise spatiotemporal control of protein dynamics in living cells

@inproceedings{Niopek2014EngineeringLN,
  title={Engineering light-inducible nuclear localization signals for precise spatiotemporal control of protein dynamics in living cells},
  author={Dominik Niopek and Dirk Benzinger and Julia Roensch and Thomas Draebing and Pierre Wehler and Roland Eils and Barbara Di Ventura},
  booktitle={Nature communications},
  year={2014}
}
The function of many eukaryotic proteins is regulated by highly dynamic changes in their nucleocytoplasmic distribution. The ability to precisely and reversibly control nuclear translocation would, therefore, allow dissecting and engineering cellular networks. Here we develop a genetically encoded, light-inducible nuclear localization signal (LINuS) based on the LOV2 domain of Avena sativa phototropin 1. LINuS is a small, versatile tag, customizable for different proteins and cell types. LINuS… CONTINUE READING
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