Engineering key components in a synthetic eukaryotic signal transduction pathway

  title={Engineering key components in a synthetic eukaryotic signal transduction pathway},
  author={Mauricio S. Antunes and Kevin J. Morey and Neera Tewari-Singh and Tessa A. Bowen and J. Jeff Smith and Colleen T. Webb and Homme W. Hellinga and June I Medford},
  journal={Molecular Systems Biology},
  pages={270 - 270}
Signal transduction underlies how living organisms detect and respond to stimuli. A goal of synthetic biology is to rewire natural signal transduction systems. Bacteria, yeast, and plants sense environmental aspects through conserved histidine kinase (HK) signal transduction systems. HK protein components are typically comprised of multiple, relatively modular, and conserved domains. Phosphate transfer between these components may exhibit considerable cross talk between the otherwise apparently… 
Developing a synthetic signal transduction system in plants.
Programmable Ligand Detection System in Plants through a Synthetic Signal Transduction Pathway
This work describes assembly and function of a complete synthetic signal transduction pathway in plants that links input from computationally re-designed periplasmic binding proteins to a visual response and may allow plants to serve as a simple and inexpensive means to monitor human surroundings for substances such as pollutants, explosives, or chemical agents.
Transplantation of prokaryotic two-component signaling pathways into mammalian cells
It is found that coexpression of HKs and their cognate RRs in cultured mammalian cells is necessary and sufficient to strongly induce gene expression even in the absence of pathways’ chemical triggers in the medium, and their components can be used for large-scale synthetic gene circuits.
Crosstalk between endogenous and synthetic components – synthetic signaling meets endogenous components
In this review, the design and function of two partially synthetic signaling pathways for use in plants is discussed and observed interactions (crosstalk) with endogenous signaling components are described.
Synthetic biosensing systems.
Engineering Signal Transduction Pathways
Synthetic gene networks in plant systems.
This chapter gives an overview of the existing plant genetic networks and describes in detail the application of two systems for inducible gene expression, the ethanol-inducible system and the dexamethasone-dependent nuclear translocation of the GOI::GR protein.
Synthetic biology of cell signaling
While creating synthetic signaling cascades is perhaps one of the most challenging tasks in synthetic biology, potential implications can be far-reaching and include new tools for programming cells and tissues, artificial developmental processes, and therapeutic tools.
Strategies for protein synthetic biology
An engineering framework is outlined for the connection of reusable protein interaction devices into self-sufficient circuits to ‘refacture’ protein complexity into well-defined exchangeable devices for predictive engineering.


Specificity in two-component signal transduction pathways.
The cellular and molecular mechanisms that dictate the specificity of two-component signaling pathways are reviewed, including the role of phosphoryl group transfer, which can then effect changes in cellular physiology, often by regulating gene expression.
Evolution of two-component signal transduction.
The structural and functional similarity between TCST kinases and eukaryotic protein kinases raises the possibility of a distant evolutionary relationship.
Fungal Histidine Kinases
Because some phosphorelay proteins are essential for virulence of microbial pathogens, including the yeast fungus Candida albicans, novel antibiotics targeted to phosphorelays may be effective against eukaryotic pathogens without causing host cell damage.
Histidine protein kinases: key signal transducers outside the animal kingdom
A large family of signal-transduction enzymes that autophosphorylate on a conserved histidine residue are found, which are important for multiple functions in bacteria, including chemotaxis and quorum sensing, and in eukaryotes, including hormone-dependent developmental processes.
Ethylene signal transduction.
Data indicate that the site of ethylene perception is at the endoplasmic reticulum and point to the importance of protein complexes in mediating the initial steps in ethylene signal transduction.
Two-Component Phosphorelay Signal Transduction Systems in Plants: from Hormone Responses to Circadian Rhythms
  • T. Mizuno
  • Environmental Science
    Bioscience, biotechnology, and biochemistry
  • 2005
In this review, recent progress with regard to studies on TCS in higher plants will be discussed, focusing particularly on the model higher plant Arabidopsis thaliana.
A subset of Arabidopsis AP2 transcription factors mediates cytokinin responses in concert with a two-component pathway.
The evolutionarily ancient two-component system that is used by cytokinin branches to incorporate a unique family of plant-specific transcription factors is revealed.
Identification of CRE1 as a cytokinin receptor from Arabidopsis
Evidence is provided that cytokinins can activate CRE1 to initiate phosphorelay signalling, and this work identifies Arabidopsis cre1 (cytokinin response 1) mutants, which exhibited reduced responses to cytokinin responses.
Plant two-component systems: principles, functions, complexity and cross talk
This review summarizes the present knowledge about the principles and functions of two-component systems in higher plants and addresses several critical points with respect to cross talk, signal integration and specificity.