Engineering improved ethanol production in Escherichia coli with a genome-wide approach.

@article{Woodruff2013EngineeringIE,
  title={Engineering improved ethanol production in Escherichia coli with a genome-wide approach.},
  author={Lauren B A Woodruff and Nanette R. Boyle and Ryan T. Gill},
  journal={Metabolic engineering},
  year={2013},
  volume={17},
  pages={1-11}
}
A key challenge to the commercial production of commodity chemical and fuels is the toxicity of such molecules to the microbial host. While a number of studies have attempted to engineer improved tolerance for such compounds, the majority of these studies have been performed in wild-type strains and culturing conditions that differ considerably from production conditions. Here we applied the multiscalar analysis of library enrichments (SCALEs) method and performed a growth selection in an… CONTINUE READING