Engineering human pluripotent stem cells into a functional skeletal muscle tissue

  title={Engineering human pluripotent stem cells into a functional skeletal muscle tissue},
  author={Lingjun Rao and Ying Qian and Alastair Khodabukus and Thomas J. Ribar and Nenad Bursac},
  booktitle={Nature Communications},
The generation of functional skeletal muscle tissues from human pluripotent stem cells (hPSCs) has not been reported. Here, we derive induced myogenic progenitor cells (iMPCs) via transient overexpression of Pax7 in paraxial mesoderm cells differentiated from hPSCs. In 2D culture, iMPCs readily differentiate into spontaneously contracting multinucleated myotubes and a pool of satellite-like cells endogenously expressing Pax7. Under optimized 3D culture conditions, iMPCs derived from multiple… CONTINUE READING
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