Engineering highly functional thermostable proteins using ancestral sequence reconstruction

  title={Engineering highly functional thermostable proteins using ancestral sequence reconstruction},
  author={Yosephin Gumulya and Jong-min Baek and Shun-Jie Wun and Raine E. S. Thomson and Kurt L. Harris and D. Hunter and James B Y H Behrendorff and Justyna Kulig and Shan Zheng and X. Wu and B. Wu and J. E. Stok and J. D. De Voss and G. Schenk and U. Jurva and S. Andersson and Emre M. Isin and M. Bod{\'e}n and L. Guddat and E. Gillam},
  journal={Nature Catalysis},
  • Yosephin Gumulya, Jong-min Baek, +17 authors E. Gillam
  • Published 2018
  • Chemistry
  • Nature Catalysis
  • Commercial biocatalysis requires robust enzymes that can withstand elevated temperatures and long incubations. Ancestral reconstruction has shown that pre-Cambrian enzymes were often much more thermostable than extant forms. Here, we resurrect ancestral enzymes that withstand ~30 °C higher temperatures and ≥100 times longer incubations than their extant forms. This is demonstrated on animal cytochromes P450 that stereo- and regioselectively functionalize unactivated C–H bonds for the synthesis… CONTINUE READING

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