Engineering highly functional thermostable proteins using ancestral sequence reconstruction

@article{Gumulya2018EngineeringHF,
  title={Engineering highly functional thermostable proteins using ancestral sequence reconstruction},
  author={Yosephine Gumulya and Jong-min Baek and Shun Jie Wun and Raine E. S. Thomson and Kurt L Harris and Dominic J.B. Hunter and James B. Y. H. Behrendorff and Justyna K. Kulig and Shan Zheng and Xuemin Wu and B Wu and Jeanette E. Stok and James J. De Voss and Gerhard Schenk and Ulrik Jurva and Shalini Andersson and Emre Mehmet Isin and Mikael Bod{\'e}n and Luke W. Guddat and Elizabeth M. J. Gillam},
  journal={Nature Catalysis},
  year={2018},
  volume={1},
  pages={878-888}
}
Commercial biocatalysis requires robust enzymes that can withstand elevated temperatures and long incubations. Ancestral reconstruction has shown that pre-Cambrian enzymes were often much more thermostable than extant forms. Here, we resurrect ancestral enzymes that withstand ~30 °C higher temperatures and ≥100 times longer incubations than their extant forms. This is demonstrated on animal cytochromes P450 that stereo- and regioselectively functionalize unactivated C–H bonds for the synthesis… Expand
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