Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer

Abstract

BACKGROUND Variants of the green fluorescent protein (GFP) with different colors would be very useful for simultaneous comparisons of multiple protein fates, developmental lineages and gene expression levels. The simplest way to shift the emission color of GFP is to substitute histidine or tryptophan for the tyrosine in the chromophore, but such blue… (More)
DOI: 10.1016/S0960-9822(02)00450-5

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@article{Heim1996EngineeringGF, title={Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer}, author={Roger Heim and Roger Tsien}, journal={Current Biology}, year={1996}, volume={6}, pages={178-182} }