Engineering fluorescent protein substrates for the AAA+ Lon protease.

  title={Engineering fluorescent protein substrates for the AAA+ Lon protease.},
  author={Matthew L. Wohlever and Andrew R. Nager and Tania A. Baker and Robert T. Sauer},
  journal={Protein engineering, design & selection : PEDS},
  volume={26 4},
AAA+ proteases, such as Escherichia coli Lon, recognize protein substrates by binding to specific peptide degrons and then unfold and translocate the protein into an internal degradation chamber for proteolysis. For some AAA+ proteases, attaching specific degrons to the N- or C-terminus of green fluorescent protein (GFP) generates useful substrates, whose unfolding and degradation can be monitored by loss of fluorescence, but Lon fails to degrade appropriately tagged GFP variants at a… CONTINUE READING