Engineering cytoplasmic male sterility via the chloroplast genome by expression of {beta}-ketothiolase.

@article{Ruiz2005EngineeringCM,
  title={Engineering cytoplasmic male sterility via the chloroplast genome by expression of \{beta\}-ketothiolase.},
  author={Oscar N Ruiz and Henry Daniell},
  journal={Plant physiology},
  year={2005},
  volume={138 3},
  pages={1232-46}
}
While investigating expression of the polydroxybutyrate pathway in transgenic chloroplasts, we addressed the specific role of beta-ketothiolase. Therefore, we expressed the phaA gene via the chloroplast genome. Prior attempts to express the phaA gene in transgenic plants were unsuccessful. We studied the effect of light regulation of the phaA gene using the psbA promoter and 5' untranslated region, and evaluated expression under different photoperiods. Stable transgene integration into the… CONTINUE READING
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