Engineering artificial antigen-presenting cells to express a diverse array of co-stimulatory molecules.

@article{Suhoski2007EngineeringAA,
  title={Engineering artificial antigen-presenting cells to express a diverse array of co-stimulatory molecules.},
  author={Megan M. Suhoski and Tatiana N. Golovina and Nicole A. Aqui and Victoria C. Tai and Angel Varela-Rohena and Michael C Milone and Richard G Carroll and James L Riley and Carl H June},
  journal={Molecular therapy : the journal of the American Society of Gene Therapy},
  year={2007},
  volume={15 5},
  pages={981-8}
}
To facilitate the therapeutic application of antigen-presenting cells (APCs), we have developed a cell-based artificial APC (aAPC) system by engineering K562 cells with lentiviruses to direct the stable expression and secretion of a variety of co-stimulatory molecules and cytokines. Here we report the use of a combinatorial lentiviral gene transfer approach to achieve long-term stable expression of at least seven genes in the K562 parental cell line. Expression of various combinations of genes… CONTINUE READING

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