Engineering and characterization of a superfolder green fluorescent protein

@article{Pedelacq2006EngineeringAC,
  title={Engineering and characterization of a superfolder green fluorescent protein},
  author={J. Pedelacq and S. Cabantous and T. Tran and T. Terwilliger and G. Waldo},
  journal={Nature Biotechnology},
  year={2006},
  volume={24},
  pages={79-88}
}
Existing variants of green fluorescent protein (GFP) often misfold when expressed as fusions with other proteins. We have generated a robustly folded version of GFP, called 'superfolder' GFP, that folds well even when fused to poorly folded polypeptides. Compared to 'folding reporter' GFP, a folding-enhanced GFP containing the 'cycle-3' mutations and the 'enhanced GFP' mutations F64L and S65T, superfolder GFP shows improved tolerance of circular permutation, greater resistance to chemical… Expand

Paper Mentions

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