Engineering and analysis of a Saccharomyces cerevisiae strain that uses formaldehyde as an auxiliary substrate.

  title={Engineering and analysis of a Saccharomyces cerevisiae strain that uses formaldehyde as an auxiliary substrate.},
  author={Richard J. S. Baerends and Erik A. F. de Hulster and Jan-Maarten A. Geertman and Jean-Marc Daran and Antonius J. A. van Maris and Marten van der Klei Veenhuis and Ida J van der Klei and Jack T. Pronk},
  journal={Applied and environmental microbiology},
  volume={74 10},
We demonstrated that formaldehyde can be efficiently coutilized by an engineered Saccharomyces cerevisiae strain that expresses Hansenula polymorpha genes encoding formaldehyde dehydrogenase (FLD1) and formate dehydrogenase (FMD), in contrast to wild-type strains. Initial chemostat experiments showed that the engineered strain coutilized formaldehyde with glucose, but these mixed-substrate cultures failed to reach steady-state conditions and did not exhibit an increased biomass yield on glucose… CONTINUE READING

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Functional analysis of structural genes for NAD -dependent formate dehydrogenase in Saccharomyces cerevisiae

K. M. Overkamp, P. Kötter, +4 authors J. T. Pronk
Yeast • 2002
View 14 Excerpts
Highly Influenced

Estudos de microorganismos encocentrados em leitos de despéjos de caldas de destilarias de Pernambuco

J.O.F. Morais, M.H.D. Maia
II. Uma nova espécie de Hansenula, H. polymorpha. Anais de Escola Superior de Quimica, Universidade do Recife • 1959
View 4 Excerpts
Highly Influenced

DNA molecules coding for FMDH control regions and structured gene for protein having FMDH activity and their uses. European patent EP0299108 (AU2443488)

C. P. Hollenberg, Z. A. Janowicz
View 10 Excerpts
Highly Influenced

Metabolic engineering of Saccharomyces cerevisiae microbial cell factories for succinic acid

J. M. Otero, L. Olsson, J. Nielsen
J. Biotechnol • 2007
View 1 Excerpt


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