Engineering and analysis of a Saccharomyces cerevisiae strain that uses formaldehyde as an auxiliary substrate.

@article{Baerends2008EngineeringAA,
  title={Engineering and analysis of a Saccharomyces cerevisiae strain that uses formaldehyde as an auxiliary substrate.},
  author={Richard J. S. Baerends and Erik A. F. de Hulster and Jan-Maarten A. Geertman and Jean-Marc Daran and Antonius J. A. van Maris and Marten van der Klei Veenhuis and Ida J van der Klei and Jack T. Pronk},
  journal={Applied and environmental microbiology},
  year={2008},
  volume={74 10},
  pages={3182-8}
}
We demonstrated that formaldehyde can be efficiently coutilized by an engineered Saccharomyces cerevisiae strain that expresses Hansenula polymorpha genes encoding formaldehyde dehydrogenase (FLD1) and formate dehydrogenase (FMD), in contrast to wild-type strains. Initial chemostat experiments showed that the engineered strain coutilized formaldehyde with glucose, but these mixed-substrate cultures failed to reach steady-state conditions and did not exhibit an increased biomass yield on glucose… CONTINUE READING

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