Engineering and Selection of Shuffled AAV Genomes: A New Strategy for Producing Targeted Biological Nanoparticles.

@article{Li2008EngineeringAS,
  title={Engineering and Selection of Shuffled AAV Genomes: A New Strategy for Producing Targeted Biological Nanoparticles.},
  author={Wuping Li and Aravind Asokan and Zhijian Wu and Terry van Dyke and Nina Diprimio and Jarrod S. Johnson and Lakshmanan Govindaswamy and Mavis Agbandje-McKenna and Stefan W. Leichtle and D Eugene Redmond and Thomas J Mccown and Kimberly B Petermann and Norman E Sharpless and Richard Jude Samulski},
  journal={Molecular therapy : the journal of the American Society of Gene Therapy},
  year={2008},
  volume={16 7},
  pages={
          1252-1260
        }
}
We report a DNA shuffling-based approach for developing cell type-specific vectors through directed evolution. Capsid genomes of adeno-associated virus (AAV) serotypes 1-9 were randomly fragmented and reassembled using PCR to generate a chimeric capsid library. A single infectious clone (chimeric-1829) containing genome fragments from AAV1, 2, 8, and 9 was isolated from an integrin minus hamster melanoma cell line previously shown to have low permissiveness to AAV. Molecular modeling studies… CONTINUE READING

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