Engineering an enzyme by site-directed mutagenesis to be resistant to chemical oxidation.

@article{Estell1985EngineeringAE,
  title={Engineering an enzyme by site-directed mutagenesis to be resistant to chemical oxidation.},
  author={David A. Estell and Thomas P. Graycar and James A. Wells},
  journal={The Journal of biological chemistry},
  year={1985},
  volume={260 11},
  pages={
          6518-21
        }
}
Site-directed mutagenesis can be employed to alter activity critical residues in proteins which are susceptible to chemical oxidation. Previous studies have implicated methionine 222 as a primary site for oxidative inactivation of subtilisin (Stauffer, C. E., and Etson, D. (1969) J. Biol. Chem. 244, 5333-5338). Because of uncertainties in predicting which amino acid would be the optimal substitute for methionine 222, we prepared all 19 amino acid substitutions at this site in the cloned… CONTINUE READING

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