Engineering an allosteric transcription factor to respond to new ligands

  title={Engineering an allosteric transcription factor to respond to new ligands},
  author={N. Denise Taylor and Alexander S. Garruss and Rocco Moretti and Sum Yin Chan and Mark A. Arbing and Duilio Cascio and Jameson K. Rogers and Farren J Isaacs and Sriram Kosuri and David P. Baker and Stanley Fields and George M. Church and Srivatsan Raman},
  journal={Nature Methods},
Genetic regulatory proteins inducible by small molecules are useful synthetic biology tools as sensors and switches. Bacterial allosteric transcription factors (aTFs) are a major class of regulatory proteins, but few aTFs have been redesigned to respond to new effectors beyond natural aTF-inducer pairs. Altering inducer specificity in these proteins is difficult because substitutions that affect inducer binding may also disrupt allostery. We engineered an aTF, the Escherichia coli lac repressor… CONTINUE READING
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