Engineering an NADPH/NADP+ Redox Biosensor in Yeast.

@article{Zhang2016EngineeringAN,
  title={Engineering an NADPH/NADP+ Redox Biosensor in Yeast.},
  author={Jie Zhang and Nikolaus Sonnenschein and Thomas P B Pihl and Kasper Rud Pedersen and Michael Krogh Jensen and Jay D. Keasling},
  journal={ACS synthetic biology},
  year={2016},
  volume={5 12},
  pages={
          1546-1556
        }
}
Genetically encoded biosensors have emerged as powerful tools for timely and precise in vivo evaluation of cellular metabolism. In particular, biosensors that can couple intercellular cues with downstream signaling responses are currently attracting major attention within health science and biotechnology. Still, there is a need for bioprospecting and engineering of more biosensors to enable real-time monitoring of specific cellular states and controlling downstream actuation. In this study, we… 
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References

SHOWING 1-10 OF 57 REFERENCES
NADH-dependent biosensor in Saccharomyces cerevisiae: principle and validation at the single cell level
TLDR
The designed system successfully allowed for monitoring perturbations in the cellular redox metabolism caused by environmental changes, or by heterologous gene expression, and displayed high resolution in distinguishing cytosolic NADH oxidation capacity and hence has potential to be used for high-throughput screening based on the fluorescence of single cells.
SoxR as a single-cell biosensor for NADPH-consuming enzymes in Escherichia coli.
TLDR
An ultra-high-throughput screening system for NADPH-dependent enzymes, such as stereospecific alcohol dehydrogenases, was established and was used successfully to screen a mutant LbAdh library for variants showing improved activity with the substrate 4-methyl-2-pentanone.
Engineered Saccharomycescerevisiae Strain BioS‐OS1/2, for the Detection of Oxidative Stress
TLDR
It is demonstrated that the engineered BioS‐OS yeast strain can detect ROI‐generating oxidative stress and validate the use of this prototypic strain for the development of a biosensor to detect and monitor oxidative stress factors during space and high altitude flights.
A Novel Fluorescent Sensor Protein for Visualization of Redox States in the Cytoplasm and in Peroxisomes
TLDR
Interestingly, it was observed that the redox state of the cytosol of cell mutants for peroxisome assembly, regarded as models for a neurological metabolic disorder, was more reductive than that of the wild-type cells in yeast and CHO cells.
Redox control of AP-1-like factors in yeast and beyond
TLDR
In yeast members of the AP-1 family of proteins are required for the transcriptional response to oxidative stress and many of the features appear to be evolutionarily conserved and consequently the study of model organisms, such as yeast, will have widespread utility.
...
1
2
3
4
5
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