Engineering alternative isobutanol production platforms

@inproceedings{FelpetoSantero2015EngineeringAI,
  title={Engineering alternative isobutanol production platforms},
  author={Carmen Felpeto-Santero and Antonia Rojas and Marta Tortajada and Beatriz Gal{\'a}n and Daniel Ram{\'o}n and J{\'e}ssica Leite Garcia},
  booktitle={AMB Express},
  year={2015}
}
A synthetic inducible operon (IbPSO) expressing alsS, ilvC, ilvD and kivD genes encoding a pathway capable to transform pyruvate into 2-isobutyraldehyde has been designed and two recombinant plasmids named pIZIbPSO and p424IbPSO were constructed. The IbPSO containing plasmids can generate in a single transformation event new recombinant isobutanol producer strains and are useful for testing as suitable hosts wild type bacteria in different culture media. In this way we found that Shimwellia… CONTINUE READING
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