Engineering a three-cysteine, one-histidine ligand environment into a new hyperthermophilic archaeal Rieske-type [2Fe-2S] ferredoxin from Sulfolobus solfataricus.

@article{Kounosu2004EngineeringAT,
  title={Engineering a three-cysteine, one-histidine ligand environment into a new hyperthermophilic archaeal Rieske-type [2Fe-2S] ferredoxin from Sulfolobus solfataricus.},
  author={Asako Kounosu and Zhongrui Li and Nathaniel J. Cosper and Jacob E. Shokes and Robert Scott and Takeo Imai and Akio Urushiyama and Toshio Iwasaki},
  journal={The Journal of biological chemistry},
  year={2004},
  volume={279 13},
  pages={12519-28}
}
We heterologously overproduced a hyperthermostable archaeal low potential (E(m) = -62 mV) Rieske-type ferredoxin (ARF) from Sulfolobus solfataricus strain P-1 and its variants in Escherichia coli to examine the influence of ligand substitutions on the properties of the [2Fe-2S] cluster. While two cysteine ligand residues (Cys(42) and Cys(61)) are essential for the cluster assembly and/or stability, the contributions of the two histidine ligands to the cluster assembly in the archaeal Rieske… CONTINUE READING