Engineering a reduced Escherichia coli genome.

@article{Kolisnychenko2002EngineeringAR,
  title={Engineering a reduced Escherichia coli genome.},
  author={Vitaliy Kolisnychenko and Guy Plunkett and Christopher D. Herring and Tam{\'a}s Feh{\'e}r and J{\'a}nos P{\'o}sfai and Frederick R. Blattner and Gy{\"o}rgy P{\'o}sfai},
  journal={Genome research},
  year={2002},
  volume={12 4},
  pages={640-7}
}
Our goal is to construct an improved Escherichia coli to serve both as a better model organism and as a more useful technological tool for genome science. We developed techniques for precise genomic surgery and applied them to deleting the largest K-islands of E. coli, identified by comparative genomics as recent horizontal acquisitions to the genome. They are loaded with cryptic prophages, transposons, damaged genes, and genes of unknown function. Our method leaves no scars or markers behind… CONTINUE READING

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