Engineering a high-affinity scaffold for non-chromatographic protein purification via intein-mediated cleavage.

@article{Liu2012EngineeringAH,
  title={Engineering a high-affinity scaffold for non-chromatographic protein purification via intein-mediated cleavage.},
  author={Fang Liu and Shen-Long Tsai and Bhawna Madan and Wilfred Chen},
  journal={Biotechnology and bioengineering},
  year={2012},
  volume={109 11},
  pages={
          2829-35
        }
}
While protein purification has long been dominated by standard chromatography, the relatively high cost and complex scale-up have promoted the development of alternative non-chromatographic separation methods. Here we developed a new non-chromatographic affinity method for the purification of proteins expressed in Escherichia coli. The approach is to genetically fuse the target proteins with an affinity tag. Direct purification and recovery can be achieved using a thermo-responsive elastin-like… CONTINUE READING
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