Engineering a genetically-encoded SHG chromophore by electrostatic targeting to the membrane

  title={Engineering a genetically-encoded SHG chromophore by electrostatic targeting to the membrane},
  author={Yuka Jinno and Keiko Shoda and Emiliano Rial-Verde and Rafael Yuste and Atsushi Miyawaki and Hidekazu Tsutsui},
  journal={Frontiers in Molecular Neuroscience},
Although second harmonic generation (SHG) microscopy provides unique imaging advantages for voltage imaging and other biological applications, genetically-encoded SHG chromophores remain relatively unexplored. SHG only arises from non-centrosymmetric media, so an anisotropic arrangement of chromophores is essential to provide strong SHG signals. Here, inspired by the mechanism by which K-Ras4B associates with plasma membranes, we sought to achieve asymmetric arrangements of chromophores at the… 

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